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Related Experiment Videos

Apolipoprotein E-enriched lipoprotein subclasses in normolipidemic subjects.

J C Gibson, A Rubinstein, P R Bukberg

    Journal of Lipid Research
    |July 1, 1983
    PubMed
    Summary

    Researchers identified three distinct subclasses of apolipoprotein E (apoE)-containing lipoproteins in human plasma using molecular sieve chromatography. This method avoids ultracentrifugation, offering a new way to study apoE lipoprotein associations.

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    Area of Science:

    • Biochemistry
    • Lipid Metabolism
    • Analytical Chemistry

    Background:

    • Apolipoprotein E (apoE) plays a crucial role in lipoprotein metabolism and transport.
    • Understanding apoE's association with different lipoprotein subclasses is essential for diagnosing and treating lipid disorders.
    • Traditional methods like ultracentrifugation can be time-consuming and may alter lipoprotein structures.

    Purpose of the Study:

    • To objectively define the lipoprotein association of apoE without using ultracentrifugation.
    • To describe distinct apoE-containing lipoprotein subclasses in normal human plasma.
    • To characterize the size and density of these apoE subclasses.

    Main Methods:

    • Lipoproteins from whole plasma were fractionated using continuous molecular sieve chromatography with 4% agarose.

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  • The elution profile of apoE was compared with apoB, apoA-I, cholesterol, and triglyceride.
  • Chromatography was performed on fractions obtained after ultracentrifugation at different densities to confirm findings.

    • Main Results:

    • All apoE in normal subjects is associated with lipoproteins and exists in three discrete subfractions.
    • Fraction I, the largest, is a subclass of very-low-density lipoproteins (VLDL).
    • Fractions II and III are distinct in size from major cholesterol-carrying lipoproteins, with Fraction II intermediate between VLDL and LDL, and Fraction III larger than HDL but smaller than LDL.

    Conclusions:

    • Molecular sieve chromatography effectively characterizes apoE-lipoprotein subclasses without ultracentrifugation.
    • Three distinct apoE-lipoprotein subclasses exist in normal plasma, differing in size.
    • These findings provide a novel method for studying apoE distribution and have implications for understanding lipid metabolism.