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Ca+2-accumulating components in developing skeletal muscle.

C M Smalls, D Goode

    Journal of Morphology
    |February 1, 1977
    PubMed
    Summary
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    Calcium accumulation in skeletal muscle begins during embryonic development. Key sites, sarcoplasmic reticulum and I-bands, develop at different times, suggesting a role in muscle contraction regulation.

    Area of Science:

    • Cell Biology
    • Muscle Physiology
    • Developmental Biology

    Background:

    • Skeletal muscle contraction relies on precise calcium (Ca+2) regulation.
    • Understanding the developmental origins of calcium-handling components is crucial for muscle physiology.

    Purpose of the Study:

    • To investigate the ultrastructural localization of Ca+2 in developing chick skeletal muscle.
    • To determine the developmental timeline of calcium-accumulating sites within muscle cells.

    Main Methods:

    • Utilized oxalate and pyroantimonate techniques for Ca+2 localization.
    • Examined ultrathin sections of embryonic and post-embryonic chick pectoral and sartorius muscles.
    • Employed electron microscopy to identify distinct cellular components accumulating Ca+2.

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    Main Results:

    • Identified two primary Ca+2 accumulation sites: sarcoplasmic reticulum terminal cisternae and I-band specific lines.
    • Observed calcium oxalate-accumulating vesicles in myotubes by the twelfth day of embryonic incubation.
    • Detected Ca+2 accumulation at myofibrillar I-band sites between the fourteenth and seventeenth days of incubation.

    Conclusions:

    • Calcium-accumulating components form during embryonic development in skeletal muscle.
    • The sarcoplasmic reticulum is an early site of Ca+2 accumulation, preceding I-band accumulation.
    • I-band Ca+2-binding components are added later, likely for contraction regulation, not myofibril formation.