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Molecular cloning of the c-fms locus and its assignment to human chromosome 5
Journal of Virology
|December 1, 1983
Abstract:
Molecular clones of the retroviral oncogene v-fms were used to isolate recombinant bacteriophages containing c-fms proto-oncogene sequences from a human placental DNA library. Viral and cellular fms sequences were used in Southern blotting experiments with a panel of 32 human X mouse somatic cell hybrids to assign the human c-fms proto-oncogene to human chromosome 5.
Insights
Researchers identified the human c-fms proto-oncogene location. Using molecular clones and DNA analysis, they pinpointed this crucial gene to human chromosome 5, advancing cancer gene research.
Area of Science:
- Molecular Biology
- Human Genetics
- Oncogene Research
Background:
- The fms gene family encodes receptors involved in cell growth and differentiation.
- Understanding the genetic basis of oncogenes is critical for cancer research.
Purpose of the Study:
- To isolate and map the human c-fms proto-oncogene.
- To determine the chromosomal location of the c-fms gene.
Main Methods:
- Isolation of c-fms sequences using molecular clones of the v-fms oncogene.
- Screening of a human placental DNA library.
- Southern blotting analysis using human X mouse somatic cell hybrids.
Main Results:
- Successfully isolated recombinant bacteriophages containing human c-fms proto-oncogene sequences.
- Assigned the human c-fms proto-oncogene to human chromosome 5 through genetic mapping.
Conclusions:
- The human c-fms proto-oncogene is located on chromosome 5.
- This localization provides a basis for further studies on the role of c-fms in human health and disease.