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Related Experiment Videos

Direct enzyme transfer from lymphocytes is specific.

I Olsen, H Muir, R Smith

    Nature
    |November 3, 1983
    PubMed
    Summary
    This summary is machine-generated.

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    Lymphocytes can transfer lysosomal enzymes, like beta-glucuronidase, to other cells through direct contact. This enzyme transfer process is specific to certain lysosomal enzymes, not all.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Immunology

    Background:

    • Lymphocytes interact with other cells in vivo and in vitro.
    • Previous studies showed lymphocytes transfer beta-glucuronidase to deficient fibroblasts.
    • This transfer requires cell-cell contact and is independent of classical endocytosis.

    Purpose of the Study:

    • To investigate the specificity of enzyme transfer between lymphocytes and fibroblasts.
    • To determine if the mechanism of enzyme transfer is limited to specific lysosomal enzymes.

    Main Methods:

    • Co-culture of lymphocytes with fibroblasts.
    • Assay of lysosomal enzyme activity in recipient fibroblasts.
    • Testing the effect of protein synthesis inhibitors.

    Related Experiment Videos

    Main Results:

    • Direct cell-cell contact is essential for enzyme transfer.
    • Inhibitors of protein synthesis block the transfer process.
    • The direct transfer of enzymes from lymphocytes to fibroblasts is restricted to specific lysosomal enzymes.

    Conclusions:

    • Lymphocytes can directly transfer certain lysosomal enzymes to fibroblasts.
    • The mechanism involves direct cell-cell contact and protein synthesis.
    • This suggests a specific pathway for lysosomal enzyme exchange between cells.