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Related Experiment Videos

Antigen-antibody complex binding and cell interaction in stimulating normal rabbit lymphocytes.

L S Soderberg

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
    |November 1, 1983
    PubMed
    Summary

    Antigen-antibody (Ag-Ab) complexes stimulate lymphocyte proliferation in vitro. This immune response requires the interaction of three specific bone marrow cell subpopulations for optimal activation.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Antigen-antibody complexes can modulate immune responses in vivo.
    • In vitro studies explore the role of antigen-antibody complexes in lymphocyte activation.

    Purpose of the Study:

    • To investigate the in vitro proliferation of lymphocytes induced by antigen-antibody complexes.
    • To identify the cell types and conditions necessary for antigen-antibody complex-mediated lymphocyte stimulation.

    Main Methods:

    • Preparation of antigen-antibody complexes in slight antigen excess using rabbit antibodies.
    • Incubation of lymphocytes from various lymphoid organs with antigen-antibody complexes.
    • Assessment of lymphocyte proliferation and binding of complexes via Fc and complement receptors.
    • Separation and recombination of bone marrow cell subpopulations (macrophage-enriched, B-cell-enriched, mIgM- cell-enriched) to determine their role in proliferation.

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    Main Results:

    • Antigen-antibody complexes induced proliferation of unprimed rabbit lymphocytes from peripheral blood, bone marrow, spleen, and lymph node, but not thymus.
    • Cells bound antigen-antibody complexes via Fc and complement receptors but required additional complex for proliferation.
    • Specific antigen addition to Ag-Ab-coated cells triggered proliferation, suggesting ligand cross-linking.
    • Proliferation was dependent on the interaction of three bone marrow subpopulations; separated or partially combined subpopulations showed reduced responsiveness.

    Conclusions:

    • Antigen-antibody complex stimulation of lymphocytes in vitro is a complex process.
    • Optimal lymphocyte proliferation requires the cooperative interaction of macrophage, B-cell, and mIgM- cell subpopulations.
    • The findings highlight the intricate cellular requirements for immune activation by antigen-antibody complexes.