Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative studies on sunburn cell formation in mouse epidermis.

M Obata

    The Tohoku Journal of Experimental Medicine
    |August 1, 1983
    PubMed
    Summary

    Ultraviolet-C and ultraviolet-B radiation are most effective at inducing sunburn cells (SC), with shorter wavelengths being more potent. High doses of ultraviolet-A can also induce SCs and potentiate the effects of UV-B and UV-C.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Anatomical examination of the relationship between the temporomandibular joint capsule and lateral ligament: a cadaveric study.

    International journal of oral and maxillofacial surgery·2025
    Same author

    Image Gallery: Cutaneous hydrophilic polymer emboli following thoracic endovascular aortic repair.

    The British journal of dermatology·2019
    Same author

    Acral melanoma in Japan.

    The Journal of investigative dermatology·2010
    Same author

    Lack of Bcl11b tumor suppressor results in vulnerability to DNA replication stress and damages.

    Oncogene·2007
    Same author

    Increased susceptibility to apoptosis in CD45(+) myeloma cells accompanied by the increased expression of VDAC1.

    Oncogene·2005
    Same author

    The effect of basic fibroblast growth factor on the regeneration of guinea pig olfactory epithelium.

    European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·2002

    Area of Science:

    • Photobiology
    • Dermatology
    • Cellular Biology

    Background:

    • Sunburn cells (SC) are indicators of epidermal damage from ultraviolet (UV) radiation.
    • Understanding the differential effects of UV-A, UV-B, and UV-C is crucial for photoprotection strategies.

    Purpose of the Study:

    • To quantitatively assess sunburn cell formation induced by different UV wavelengths (UV-A, UV-B, UV-C).
    • To determine the dose-response relationships and time courses for SC formation.
    • To investigate the combined effects of different UV types on SC induction.

    Main Methods:

    • Quantitative analysis of sunburn cell formation using mouse epidermal sheets.
    • Exposure to monochromatic ultraviolet-A (UV-A), ultraviolet-B (UV-B), and ultraviolet-C (UV-C) at various doses.
    • Utilized NaBr split and Hematoxylin & Eosin (H&E) staining for histological assessment.

    Main Results:

    • Action spectra revealed wavelengths <300 nm were most effective for SC formation.
    • UV-A (360 nm) required approximately 600 times more energy than UV-B (300 nm) for equivalent SC induction.
    • UV-B and UV-C exhibited a logarithm-like dose-response for SC formation.
    • Peak SC formation occurred at 18h (UV-C), 24h (UV-B), and 30h (UV-A) post-irradiation.
    • High-dose UV-A enhanced SC formation induced by UV-B or UV-C.

    Conclusions:

    • UV-C and UV-B are significantly more potent inducers of sunburn cells than UV-A.
    • UV-A can contribute to sunburn cell formation and exacerbate UV-B/UV-C-induced damage.
    • The findings provide insights into the wavelength-dependent mechanisms of UV-induced skin damage.

    Related Experiment Videos