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DNA structure: evidence from electron microscopy.

J D Griffith

    Science (New York, N.Y.)
    |August 11, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Electron microscopy revealed that RNA-DNA hybrids have a rise of 2.5 angstroms per base pair, while DNA duplexes show a 2.9 angstrom rise, challenging existing chromatin models.

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    Area of Science:

    • Molecular Biology
    • Biophysics
    • Structural Biology

    Background:

    • Electron microscopy is a key technique for visualizing DNA structure.
    • Established models of DNA conformation (e.g., B-form) assume specific base pair rise values.

    Purpose of the Study:

    • To accurately measure the contour lengths of phiX174 DNA duplex and RNA-DNA hybrid molecules using electron microscopy.
    • To reassess chromatin structure parameters derived from electron microscopy data.

    Main Methods:

    • Utilized multiple standard electron microscopic techniques.
    • Measured contour lengths of phiX174 DNA duplex and RNA-DNA hybrid molecules.

    Main Results:

    • RNA-DNA hybrids exhibited a rise of 2.5-2.6 angstroms per base pair, consistent with the A conformation.

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  • phiX174 DNA duplexes measured a rise of 2.9 angstroms, deviating significantly from the classic B-form's 3.4 angstroms.
  • These findings indicate a potential discrepancy in current electron microscopy-based chromatin structure parameters.

    • Conclusions:

    • Electron microscopy measurements suggest DNA may adopt conformations with a lower base pair rise than previously assumed.
    • Existing chromatin structure models relying on a 3.4 angstrom rise may require re-evaluation.
    • The study discusses the possibility of DNA in solution having a 2.9 angstrom rise and 10.5 base pairs per turn.