Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in adenylate cyclase activity in dog livers during endotoxic shock.

S Ghosh, M S Liu

    The American Journal of Physiology
    |November 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Three cases of laryngeal myxoma].

    Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2026
    Same author

    [The interaction of workplace noise, body mass index and systemic inflammatory response on hypertension].

    Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2025
    Same author

    [Attention should be paid to the importance of genetic testing in clinical practice of amyotrophic lateral sclerosis].

    Zhonghua yi xue za zhi·2023
    Same author

    [Rapid promyelocytic blast crisis of chronic myeloid leukemia with PML-RARα fusion gene: a case report and literature review].

    Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2023
    Same author

    Time resolved absolute extreme ultraviolet radiation measurement on the ENN XuanLong-50 spherical tokamak.

    The Review of scientific instruments·2021
    Same author

    Toroidal soft x-ray array on the EXL-50 spherical tokamak.

    The Review of scientific instruments·2021
    Same journal

    Blood coagulation in fish.

    The American journal of physiology·2011
    Same journal

    Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

    The American journal of physiology·2010
    Same journal

    The inactivation of placental toxin by human serum.

    The American journal of physiology·2010
    Same journal

    Adrenal function following ovariectomy in the rat.

    The American journal of physiology·2010
    Same journal

    Capillary permeability; perfusion of frog and guinea pig hind limbs.

    The American journal of physiology·2010
    Same journal

    Evaluation of protective measures against sunburn.

    The American journal of physiology·2010
    See all related articles

    Endotoxin significantly inhibits adenylate cyclase activity in dog liver plasma membranes. This finding helps explain how endotoxin affects glucose metabolism and homeostasis during shock.

    Area of Science:

    • Biochemistry
    • Cellular Physiology

    Background:

    • Adenylate cyclase is a key enzyme in cellular signaling pathways.
    • The adenylate cyclase-adenosine 3',5'-cyclic monophosphate (cAMP) system regulates hepatic carbohydrate metabolism.
    • Endotoxin administration can disrupt normal physiological processes, particularly during shock.

    Purpose of the Study:

    • To investigate the impact of endotoxin on adenylate cyclase activity in dog liver plasma membranes.
    • To determine if endotoxin affects basal and stimulated adenylate cyclase activity.
    • To elucidate the role of endotoxin in altering hepatic glucose homeostasis.

    Main Methods:

    • Studied basal, fluoride-, guanine nucleotide-, isoproterenol-, and glucagon-stimulated adenylate cyclase activities.
    • Administered endotoxin both in vivo and in vitro to dog liver plasma membranes.

    Related Experiment Videos

  • Quantified changes in enzyme activity following endotoxin exposure.

    • Main Results:

    • In vivo endotoxin administration decreased basal, fluoride-, guanine nucleotide-, isoproterenol-, and glucagon-stimulated adenylate cyclase activities by 61%, 62%, 69%, 83%, and 63%, respectively.
    • In vitro endotoxin exposure reduced guanine nucleotide-, isoproterenol-, and glucagon-stimulated adenylate cyclase activities by 24%, 25%, and 21%, respectively.
    • Demonstrated a significant inhibitory effect of endotoxin on the adenylate cyclase enzyme system.

    Conclusions:

    • Endotoxin exerts an inhibitory effect on dog liver plasma membrane adenylate cyclase, both in vivo and in vitro.
    • Reduced adenylate cyclase activity by endotoxin contributes to understanding altered hepatic glucose homeostasis during shock.
    • This research provides insights into the pathophysiology of endotoxin-induced metabolic disturbances.