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Related Experiment Videos

Lectin-binding domains on laminin.

C N Rao, I J Goldstein, L A Liotta

    Archives of Biochemistry and Biophysics
    |November 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Carbohydrate mapping of laminin reveals distinct sugar distributions. Alpha-D-galactopyranosyl groups are concentrated in globular regions, while complex oligosaccharides are enriched in rod-shaped areas of laminin.

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    An "extension" of the carbohydrate binding specificity of concanavalin A.

    Biochimica et biophysica acta·2009

    Area of Science:

    • Glycobiology
    • Molecular Biology
    • Extracellular Matrix Research

    Background:

    • Laminin is a crucial component of basement membranes.
    • Its cross-shaped structure comprises short and long arms with globular end regions.
    • Understanding laminin's glycosylation is key to its function.

    Purpose of the Study:

    • To identify the location and nature of carbohydrate moieties on the laminin molecule.
    • To investigate the binding affinities of lectins for laminin fragments.
    • To map specific sugar residues to distinct structural domains of laminin.

    Main Methods:

    • Proteolytic fragmentation of purified laminin into distinct domains (e.g., arm-only, arm-and-globular-region-depleted fragments).
    • Lectin binding assays using a series of lectins with known sugar specificities.

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  • Hapten sugar competition assays to confirm lectin-carbohydrate interactions.

    • Main Results:

    • Alpha-D-galactopyranosyl end groups are enriched in globular end regions of laminin's short arms.
    • Alpha-D-mannopyranosyl residues are found in both globular and rod-shaped portions.
    • Complex oligosaccharides, including NANA-Gal-GlcNAc-Man structures, are enriched in rod-shaped regions.
    • Exposed N-acetyl-D-galactosaminyl end groups are scarce on laminin.

    Conclusions:

    • Laminin exhibits distinct glycosylation patterns across its structural domains.
    • Globular regions are rich in alpha-D-galactopyranosyl end groups.
    • Rod-shaped regions are enriched with specific complex oligosaccharides, suggesting domain-specific functional roles.