Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Testosterone metabolites in dog bile.

Y Yamamoto, Y Osawa, R Y Kirdani

    Steroids
    |February 1, 1978
    PubMed
    Summary

    Testosterone is primarily excreted in bile, not urine, in dogs. Researchers identified six testosterone monoglucuronide fractions and several other metabolites in bile, advancing the study of steroid metabolism.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cytogenetic and pathologic aspects of Ewing's sarcoma and neuroectodermal tumors.

    Human pathology·1992
    Same author

    Translocation (X;1)(p11.2;q21). A subtype of renal adenocarcinomas.

    Cancer genetics and cytogenetics·1992
    Same author

    Cytogenetic and molecular studies of a familial renal cell carcinoma.

    Cancer genetics and cytogenetics·1992
    Same author

    Cytogenetic and fluorescence in situ hybridization analysis of breast fibroadenomas.

    Cancer genetics and cytogenetics·1992
    Same author

    Application of fluorescence in situ hybridization in hematological disorders.

    Cancer genetics and cytogenetics·1992
    Same author

    Cytogenetic and FISH studies of abnormal X chromosomes in a patient with ANLL.

    Cancer genetics and cytogenetics·1992

    Area of Science:

    • Endocrinology
    • Metabolism Studies
    • Animal Physiology

    Background:

    • Testosterone metabolism and excretion pathways are crucial for understanding its physiological effects and potential drug interactions.
    • Biliary excretion is a significant route for steroid elimination in many species, but specific pathways for testosterone metabolites require detailed investigation.

    Purpose of the Study:

    • To investigate the excretion routes and identify the biliary metabolites of testosterone in a canine model.
    • To develop and refine methods for the analysis of testosterone conjugates and aglycones in bile.

    Main Methods:

    • Intravenous injection of radiolabeled testosterone (Testosterone-1,2-3H) into a male dog with a bile fistula.
    • Collection and analysis of bile and urine radioactivity.
    • Fractionation of biliary metabolites using DEAE-Sephadex A-25 column chromatography.
    • Isolation and purification of aglycone metabolites via Lipidex 5000 column chromatography, thin-layer chromatography (TLC), and paper chromatography.
    • Identification of metabolites using co-crystallization methods.

    Main Results:

    • Testosterone was predominantly excreted in bile (52% of the dose within 6 hours), with only 12% appearing in urine.
    • Six distinct monoglucuronide fractions of testosterone were isolated from bile, along with an unchanged fraction.
    • No other types of conjugates were detected.
    • Identified biliary aglycone metabolites included epiandrosterone, etiocholanlone, 5alpha-androstan-3beta,17beta-diol, 5beta-androstan-3alpha,17beta-diol, and 5beta-androstan-3beta,17beta-diol.

    Conclusions:

    • Bile is the primary route for testosterone excretion in dogs.
    • The study successfully identified and characterized several key biliary metabolites of testosterone, including monoglucuronides and specific androstane derivatives.
    • The developed methodologies provide a foundation for further research into steroid metabolism and biliary excretion in various animal models.

    Related Experiment Videos