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Related Experiment Videos

Counting microaggregate particles in blood.

A Suehiro, H Leinberger, J J McNamara

    Transfusion
    |May 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    This study presents a new method for measuring blood microaggregates using an electronic particle counter without hemolytic agents. Reduced saponin concentrations ensure accurate measurements and minimize artifactual microaggregate formation in stored blood samples.

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    Area of Science:

    • Hematology
    • Analytical Chemistry
    • Biomedical Engineering

    Background:

    • Blood microaggregates are critical indicators in stored blood.
    • Traditional methods for measuring microaggregates often employ hemolytic agents, which can induce artifacts.
    • Accurate quantification of microaggregates is essential for blood storage quality assessment.

    Purpose of the Study:

    • To develop and validate a method for measuring microaggregates in stored blood using an electronic particle counter.
    • To assess the impact of hemolytic agents on microaggregate formation.
    • To optimize a method that avoids artifactual microaggregate induction.

    Main Methods:

    • Utilized an electronic particle counter with two different sized apertures to measure microaggregates (12.7–80.6 micron diameter).

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  • Investigated the effect of hemolytic agents, specifically saponin, on fresh and stored blood.
  • Compared microaggregate counts in unhemolyzed versus hemolyzed blood samples with varying saponin concentrations.

    • Main Results:

    • The developed method reliably measures microaggregates within the specified size range.
    • Hemolytic agents, including standard saponin concentrations, were shown to induce microaggregate formation in fresh blood.
    • A tenfold reduction in saponin concentration achieved satisfactory hemolysis without significant microaggregate induction.
    • Hemolytic agents decreased microaggregate populations in stored blood, an effect minimized by reduced saponin concentrations.

    Conclusions:

    • A novel electronic particle counting method effectively measures blood microaggregates without inducing artifacts.
    • Optimized saponin concentrations provide reliable hemolysis while preventing artifactual microaggregate formation.
    • This method offers a more accurate assessment of microaggregates in stored blood quality control.