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Bovine placentomes contain factors which decrease progesterone secretion.

M Shemesh, W Hansel, J F Strauss

    Biology of Reproduction
    |November 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

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    Bovine placental extracts inhibit progesterone secretion by granulosa and luteal cells. This heat-stable factor, found in both maternal and fetal tissues, has molecular weights of approximately 60,000 and 30,000 daltons.

    Area of Science:

    • Reproductive Biology
    • Endocrinology
    • Biochemistry

    Background:

    • Progesterone is crucial for maintaining pregnancy.
    • Bovine placental development involves complex hormonal regulation.
    • Granulosa and luteal cells are key players in progesterone synthesis.

    Purpose of the Study:

    • To investigate the presence and nature of factors in bovine placental extracts that affect progesterone secretion.
    • To determine if these factors originate from maternal or fetal components of the placenta.
    • To characterize the biochemical properties of the identified inhibitory substance.

    Main Methods:

    • Preparation of ammonium sulfate precipitates from bovine placental cytosolic extracts (100-150 days gestation).
    • Assay of progesterone secretion by bovine granulosa and dispersed luteal cells in response to placental extracts.

    Related Experiment Videos

  • Coincubation of placental cells with luteal cells and analysis of progesterone levels.
  • Characterization of the inhibitory substance using heat stability, ether extraction, Sephadex G-100 chromatography, and isoelectric focusing.

    • Main Results:

    • Placental extracts significantly inhibited progesterone secretion in a dose-dependent manner.
    • Inhibitory activity was detected in extracts from both maternal caruncles and fetal cotyledons.
    • The inhibitory factor demonstrated heat stability and was not ether-extractable.
    • Chromatographic analysis revealed two distinct molecular weight peaks (approx. 60,000 and 30,000 daltons).
    • Isoelectric focusing identified multiple inhibitory peaks with pIs ranging from 3-5 and 6-9.

    Conclusions:

    • Bovine placenta produces a heat-stable inhibitory factor affecting progesterone synthesis.
    • This factor is present in both maternal and fetal placental tissues.
    • The substance has distinct molecular weights and isoelectric points, suggesting it may be a protein or complex of proteins.