Generating monoclonal antibodies against excised gel bands to correlate immunocytochemical and biochemical data

Two MAbs against fixed leech CNS which bind the nociceptive neurons, either the complete set or the lateral subset, yield bands on immunoblots of SDS acrylamide gels. When one of these bands is excised from a gel and used as immunogen. MAbs showing histological and biochemical properties similar to the original mAbs are obtained.