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[3H]corticosterone binding in the caudate-putamen.

C H Defiore, B B Turner

    Brain Research
    |November 14, 1983
    PubMed
    Summary

    Glucocorticoid receptors are present in the caudate-putamen of rats, detectable biochemically even after adrenalectomy. This finding contrasts with previous autoradiographic studies, suggesting a more nuanced understanding of glucocorticoid action in the brain.

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    Area of Science:

    • Neuroscience
    • Endocrinology
    • Biochemistry

    Background:

    • Autoradiographic studies suggest limited specific binding of corticosterone in the caudate-putamen.
    • Chronic glucocorticoid treatment can alter caudate-putamen biochemical parameters, including choline uptake.
    • The presence and function of glucocorticoid receptors in the caudate-putamen remain incompletely understood.

    Purpose of the Study:

    • To investigate the detectability of glucocorticoid receptors in the caudate-putamen of adrenalectomized rats using a biochemical approach.
    • To quantify specific [3H]corticosterone binding in cytosols and nuclear retention in vivo within the caudate-putamen.

    Main Methods:

    • Adrenalectomized rats were used to eliminate endogenous glucocorticoids.
    • Specific [3H]corticosterone binding in cytosols from the caudate-putamen, hippocampus, and cerebellum was measured.
    • In vivo nuclear retention of [3H]corticosterone was assessed after tail vein injection.
    • Competition experiments were performed to determine the relative affinities of various steroids for the receptor.

    Main Results:

    • The caudate-putamen exhibited significant specific [3H]corticosterone binding capacity, approximately 56% of that in the hippocampus 72 hours post-adrenalectomy.
    • The apparent dissociation constant (Kd) for [3H]corticosterone did not differ substantially between the caudate-putamen and hippocampus.
    • Cytosolic binding capacity in the caudate-putamen was comparable to the cerebellum.
    • No differences in binding capacity were observed between the hippocampus and caudate-putamen at 12 hours post-adrenalectomy.
    • Competition studies revealed similar relative steroid affinities in the caudate-putamen and hippocampus, consistent with classical glucocorticoid receptors.

    Conclusions:

    • Biochemical evidence supports the presence of functional glucocorticoid receptors in the rat caudate-putamen.
    • These receptors exhibit characteristics similar to those found in other brain regions like the hippocampus.
    • The findings suggest that the caudate-putamen is a target for glucocorticoid action, despite limited autoradiographic evidence of specific binding.

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