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Related Experiment Videos

Calcium efflux from amphibian sciatic nerve.

R E Snyder, D W O'Brien

    Canadian Journal of Physiology and Pharmacology
    |September 1, 1983
    PubMed
    Summary

    The Xenopus laevis sciatic nerve perineurium acts as a significant diffusion barrier for calcium. This sheath significantly slows calcium efflux, binding substantial amounts of the ion.

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    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biophysics

    Background:

    • The sciatic nerve's perineurial sheath plays a crucial role in regulating the nerve microenvironment.
    • Understanding calcium dynamics within the nerve is vital for comprehending neuronal function and injury response.

    Purpose of the Study:

    • To quantify the role of the perineurial sheath in calcium (45CaCl2) efflux from Xenopus laevis sciatic nerve.
    • To determine the diffusion characteristics and permeability of the perineurial sheath for calcium.

    Main Methods:

    • Intraperitoneal injection of 45CaCl2 in Xenopus laevis.
    • Measurement of calcium efflux from sciatic nerve segments with and without the perineurial sheath.
    • Mathematical modeling to determine diffusion coefficients and permeability.

    Main Results:

    • Calcium efflux from desheathed nerve showed three compartments with half-lives of ~2.4, ~30, and ~196 minutes.
    • The intact perineurial sheath significantly slowed calcium efflux, with a measured half-life of ~862 minutes.
    • The perineurium demonstrated a permeability of 3.4 x 10(-7) cm/s and bound 1-2 times the calcium concentration of plasma.

    Conclusions:

    • The perineurial sheath acts as a substantial diffusion barrier to calcium.
    • The sheath's calcium binding/sequestering capacity influences calcium homeostasis within the nerve.
    • These findings highlight the importance of the perineurium in regulating ion transport and maintaining nerve integrity.

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