The site of sulfated glycoprotein biosynthesis in rat gastric mucosa

The site of the synthesis of the sulfated glycoproteins in rat gastric mucosa using organ culture system has been studied. About 98% of the 35S radioactivity associated with the glycoproteins was found in corpus region. The sulfated glycoproteins were synthesized mainly in corpus, poorly in antrum and none in forestomach. The synthesized sulfated glycoproteins were heterogeneous by equilibrium centrifugation in CsCl density gradients. The 35S-labeled oligosaccharides released from the glycoproteins under alkaline condition were composed of 4-8 sugar residues.