Human trabecular meshwork in primary culture: a morphological and autoradiographic study

The growth and behaviour of human trabecular meshwork cells in primary culture was studied by light microscopy, time-lapse cinephotomicrography, autoradiography and electron microscopy. In all 925 explants of trabecular tissue from 132 eyes (68 +/- 14 years) were set up, and 20% produced extensive monolayers. Failure to produce primary outgrowths did not increase substantially until the eyes were over 5 days post-mortem. The latent period prior to growth was anything from a few days to 4 weeks; only then did meshwork cells label with [3H]-thymidine. The cells which grew incorporated large amounts of [3H]-leucine and this served as a useful marker to identify migratory cells. In addition they had the ultrastructural features of metabolically active cells including many mitochondria, a well-developed Golgi apparatus, abundant rough endoplasmic reticulum and prominent lysosomes. It was considered that the cells in primary culture had many features in common with 'activated' rather than normal meshwork cells.