Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Circadian variations in mouse bone marrow.

N P Aardal, O D Laerum

    Experimental Hematology
    |October 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Mouse hematopoietic stem cells exhibit significant daily rhythms, with multipotent stem cells (CFUS) and committed stem cells (CFUC) peaking at 1 PM. These circadian variations are reproducible and linked to myeloid cell counts.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Inflammation and uPAR-Expression in Colorectal Liver Metastases in Relation to Growth Pattern and Neo-adjuvant Therapy.

    Cancer microenvironment : official journal of the International Cancer Microenvironment Society·2015
    Same author

    Growth pattern of colorectal liver metastasis as a marker of recurrence risk.

    Clinical & experimental metastasis·2015
    Same author

    Comparison between ovalbumin and ovalbumin peptide 323-339 responses in allergic mice: humoral and cellular aspects.

    Scandinavian journal of immunology·2010
    Same author

    Airway inflammation and bronchial remodelling in toluene diisocyanate-exposed BALB/c mouse model.

    Scandinavian journal of immunology·2007
    Same author

    Invasiveness in vitro and biological markers in human primary glioblastomas.

    Journal of neuro-oncology·2002
    Same author

    [Konrad Birkhaug and the BCG vaccine].

    Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke·2001

    Area of Science:

    • Hematology
    • Chronobiology
    • Stem Cell Biology

    Background:

    • Hemopoiesis, the process of blood cell formation, is crucial for maintaining health.
    • Understanding daily rhythms in hemopoiesis can reveal insights into immune function and disease susceptibility.
    • Previous research suggests temporal variations in biological processes, but specific circadian patterns in hematopoietic stem cells require further elucidation.

    Purpose of the Study:

    • To investigate circadian variations in mouse hemopoiesis during different seasons.
    • To analyze the daily rhythms of multipotent stem cells (CFUS) and committed stem cells (CFUC).
    • To correlate stem cell rhythms with myeloid cell counts and assess reproducibility.

    Main Methods:

    • Spleen colony assay and agar colony technique were employed to quantify stem cells.

    Related Experiment Videos

  • Differential counting was used to assess hematopoietic cell populations.
  • Experiments were conducted in early spring and summer, with 48-hour monitoring for reproducibility.

    • Main Results:

    • Multipotent stem cells (CFUS) and committed stem cells (CFUC) showed strong circadian variations, peaking at 1300 (MET).
    • The rhythms of CFUS and CFUC were correlated with variations in myeloid cells per femur.
    • Circadian rhythms for CFUC were reproducible across seasons and over 48 hours; erythroid cell changes were less pronounced.

    Conclusions:

    • Mouse hemopoiesis, particularly stem cell populations, exhibits significant and reproducible circadian rhythms.
    • These daily variations in stem cells are linked to myeloid cell numbers, suggesting a coordinated temporal regulation.
    • The findings highlight the importance of considering circadian factors in hematological research and potential therapeutic interventions.