Effects of PGBx on glucose utilization and glycogen content of the isolated rat diaphragm

Under an environment of 100% O2, PGBx caused a significant dose dependent decrease in glucose uptake by skeletal muscle from the incubation medium, using the isolated rat diaphragm as a model system. At concentrations of PGBx below 200 micrograms/flask, however, significant alterations in the amount of glucose taken up by the tissues were not observed. The glycogen content of the tissues examined was not changed by the presence of PGBx in the incubation mixture at any of the concentrations studied. When tissues were incubated under room air, however, no PGBx-induced effects on glucose utilization or glycogen concentration were detected.