Determination of azarole plus its reduction product by HPLC

An analytical methodology for the assay of azarole plus its reduced product in plasma was developed. The method consists of oxidation of the sample with potassium ferrocyanide, extraction, and subsequent analysis by high performance liquid chromatography. Assays of prepared plasma samples demonstrated an overall precision of +/- 6.2%; accuracy ranged from -9.1% low to 5.1% high, based on mean values at several concentrations. Analysis of variance for the effects of concentration, time, and the interactive term concentration X time were all insignificant.