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O serotyping scheme for Enterobacter cloacae.

M A Gaston, C Bucher, T L Pitt

    Journal of Clinical Microbiology
    |November 1, 1983
    PubMed
    Summary
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    A new serotyping scheme for Enterobacter cloacae using heat-stable somatic antigens successfully typed 77.6% of clinical isolates. This method aids in differentiating Enterobacter cloacae strains for epidemiological studies.

    Area of Science:

    • Microbiology
    • Immunology

    Background:

    • Enterobacter cloacae is an opportunistic pathogen frequently isolated from clinical settings.
    • Accurate identification and differentiation of Enterobacter cloacae strains are crucial for infection control and epidemiological surveillance.
    • Existing methods for Enterobacter cloacae typing may have limitations in speed, specificity, or accessibility.

    Purpose of the Study:

    • To develop and validate a serotyping scheme for Enterobacter cloacae based on heat-stable somatic antigens.
    • To assess the typability of clinical isolates of Enterobacter cloacae using the developed scheme.

    Main Methods:

    • Preparation of 28 antisera in rabbits targeting heat-stable somatic antigens of Enterobacter cloacae.
    • Titration of antisera to determine agglutinin titers (greater than 640).

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  • Absorption of cross-reacting sera to enhance specificity.
  • Serotyping of 300 clinical isolates from 66 hospitals.

    • Main Results:

    • A high agglutinin titer (greater than 640) was achieved for the prepared antisera.
    • 11 out of 28 antisera required absorption due to cross-reactions.
    • 77.6% of 300 clinical isolates were successfully typable using the developed scheme.
    • 11.4% of isolates were untypable, and 11.0% were autoagglutinable.

    Conclusions:

    • The developed serotyping scheme based on heat-stable somatic antigens is effective for a significant proportion of Enterobacter cloacae clinical isolates.
    • The scheme provides a valuable tool for differentiating Enterobacter cloacae strains, aiding in epidemiological investigations.
    • Further refinement may be needed to address untypable or autoagglutinable strains.