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Dot-blotting--a novel screening assay for antibodies in hybridoma cultures

J Sternberg, P Jeppesen

    Journal of Immunological Methods
    |November 11, 1983
    PubMed

    A novel solid phase radioimmunoassay using nitrocellulose filters, is described. The method is of particular value in screening hybridoma cultures for monoclonal antibodies against chromosomal protein. Also it permits detection of antibodies directed against epitopes stable to SDS denaturation.

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