Preferential synthesis of differentiation related cytoplasmic proteins in low-serum adapted differentiated glioma cells

Rat glioma (C6) cells can be induced to morphological and biochemical differentiation by culturing in serum-deprived conditions. The induced differentiated cells were found to preferentially synthesize some distinct cytoplasmic proteins, and the rate of synthesis quantitatively correlated well with the degree of morphological differentiation, whereas the total mRNA synthesis was decreased in the differentiated cells. These findings suggest that reduced total mRNA syntheses and preferential translation of differentiation-related mRNAs are parts of the biochemical events during the differentiation process of neuroglia.