[Sedimentation of alkaline lysates of irradiated rat bone marrow cells]

A method has been developed for registration of sedimentation diagrams of alkaline lysates of mammalian cells based on measuring UV-absorption of fractions of linear sucrose gradient during its passage down the flow of UV-cord. DNA sedimentation in alkaline lysates of irradiated bone marrow cells of rats was analyzed. The ability of these cells to repair single-strand breaks during the postirradiation incubation in buffer at 37 degrees C was demonstrated. The proposed method could be applied for screening the compounds affecting the damage and repair of DNA in a cell.