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Related Experiment Videos

A collaborative calibration study of reference materials for thromboplastins.

J Hermans, A M van den Besselaar, E A Loeliger

    Thrombosis and Haemostasis
    |October 31, 1983
    PubMed
    Summary

    Five thromboplastins were calibrated against the World Health Organisation primary reference preparation. This study established calibration relationships for BCR and WHO reference materials using prothrombin time measurements.

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    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Laboratory Medicine

    Background:

    • Accurate thromboplastin calibration is crucial for reliable International Normalized Ratio (INR) determination in coagulation testing.
    • Reference materials from the European Community Bureau of Reference (BCR) and World Health Organisation (WHO) are essential for this calibration process.

    Purpose of the Study:

    • To calibrate five thromboplastin preparations, including BCR and WHO reference materials, against the WHO primary international reference preparation 67/40.
    • To establish calibration relationships for different thromboplastin tissue types (human brain, bovine, rabbit).

    Main Methods:

    • A collaborative study involving ten laboratories.
    • Calibration of five thromboplastins (three BCR, two WHO secondary) against WHO primary international reference preparation 67/40.

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  • Measurement of prothrombin times in seconds and expression of calibration relations using linear regression on the logarithms of these times.

    • Main Results:

    • Successful calibration of BCR reference materials (BCT/099, OBT/79, RBT/79) and WHO secondary international reference preparations (68/434, 70/178).
    • Characterization of thromboplastins derived from human brain, bovine, and rabbit tissue sources.
    • Demonstration of a linear relationship between the logarithms of prothrombin times for the calibrated thromboplastins.

    Conclusions:

    • The study successfully established calibration relationships for BCR and WHO reference thromboplastins.
    • The findings support the use of these reference materials for accurate thromboplastin calibration in clinical laboratories.
    • The methodology provides a basis for inter-laboratory comparability in coagulation testing.