Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Particle size distribution analysis as a rapid method to detect significant bacteriuria.

J S Baker, G M Ederer, D Mundschenk

    The American Journal of Medical Technology
    |October 1, 1983
    PubMed
    Summary

    Particle size distribution analysis (PSDA) is not sensitive enough for rapid screening of significant bacteriuria. This method requires higher bacterial counts to accurately detect infections, limiting its clinical use.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Validation of iCare IC200® rebound tonometry for intraocular pressure measurement.

    Journal francais d'ophtalmologie·2025
    Same author

    Depression diagnostics using a nonlinear mathematical oscillatory model.

    Computer methods and programs in biomedicine·2024
    Same author

    Electric Belts and Pads.

    Hall's journal of health·2022
    Same author

    Physical Activity and Sedentary Behavior of Elderly Populations during Confinement: Results from the FRENCH COVID-19 ONAPS Survey.

    Experimental aging research·2021
    Same author

    The economic costs of planting, preserving, and managing the world's forests to mitigate climate change.

    Nature communications·2020
    Same author

    The Effects of Concurrent Training Order on Satellite Cell-Related Markers, Body Composition, Muscular and Cardiorespiratory Fitness in Older Men with Sarcopenia.

    The journal of nutrition, health & aging·2020

    Area of Science:

    • Clinical Microbiology
    • Diagnostic Technology
    • Urinalysis

    Background:

    • Significant bacteriuria requires rapid and accurate detection for timely treatment.
    • Traditional methods like colony counts can be time-consuming.
    • Particle size distribution analysis (PSDA) offers a potential rapid screening alternative.

    Purpose of the Study:

    • To evaluate PSDA as a rapid screening method for significant bacteriuria.
    • To compare PSDA results with standard colony counts.
    • To determine the sensitivity and specificity of PSDA for detecting bacterial infections in urine specimens.

    Main Methods:

    • PSDA was performed using a C1000 Channelyzer linked to a Coulter Counter.
    • 800 urine specimens were analyzed, comparing PSDA screening with colony counts.

    Related Experiment Videos

  • Serial dilution colony counts were conducted on 109 culture-positive specimens.

    • Main Results:

    • PSDA demonstrated 92% specificity but only 64% sensitivity for detecting ≥10^5 colony forming units (CFU)/ml.
    • Low sensitivity was attributed to specimens with bacterial loads between 10^5 and 10^7 CFU/ml.
    • Sensitivity increased to 90% for ≥10^7 CFU/ml and 100% for ≥10^8 CFU/ml.

    Conclusions:

    • The current PSDA method exhibits insufficient sensitivity for reliable clinical screening of significant bacteriuria.
    • Higher bacterial concentrations (≥10^7 CFU/ml) improve PSDA sensitivity.
    • Further refinement of PSDA technology is needed before clinical adoption.