A method for the synthesis of D-3-phospho[U-14C]glycerate

An enzymatic method for the synthesis of radioactive D-3-phosphoglycerate from commercially available D-[U-14C]fructose 1,6-diphosphate is described. The unique aspect of this procedure is the substitution of arsenate for phosphate in the glyceraldehyde-3-phosphate dehydrogenase reaction. The 1-arseno-3-phosphoglycerate formed spontaneously hydrolyzes to form the D-3-phosphoglycerate product. The methods detailed below for the synthesis, isolation, and analysis of the 3-phospho[U-14C]glycerate product are relatively easy.