Related Experiment Video

Updated: Oct 11, 2026

RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
08:52

RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract

Published on: September 28, 2011

[R-plasmids of bacteria of the genus Pseudomonas isolated from industrial sewage]

E V Kozlova, A M Boronin

    Antibiotiki
    |October 1, 1983
    PubMed

    Insights

    Antibiotic-resistant Pseudomonas strains from sewage carry multiple resistance plasmids. These plasmids, conferring resistance to streptomycin, kanamycin, and tetracycline, can transfer between bacteria.

    Area of Science:

    • Microbiology
    • Environmental Science
    • Genetics

    Context:

    • Antibiotic manufacturing plant wastewater is a potential reservoir for antibiotic resistance genes.
    • Pseudomonas species are common environmental bacteria that can acquire and disseminate resistance mechanisms.

    Purpose:

    • To investigate the prevalence of antibiotic resistance in Pseudomonas strains isolated from antibiotic plant sewage.
    • To identify and characterize the resistance plasmids present in these strains and assess their transferability.

    Summary:

    • 132 Pseudomonas strains from untreated sewage showed high resistance to streptomycin (77%) and carbenicillin (75%), with lower resistance to kanamycin (37.5%) and tetracycline (23%).
    • Nineteen strains harbored 18 conjugative and 3 nonconjugative plasmids, with genes for streptomycin, kanamycin, and tetracycline resistance being most frequent.
    • Plasmid transfer frequency in Pseudomonas aeruginosa (PAO) ranged from 10(-3) to 10(-7) per donor cell. Plasmids belonged to Inc P groups P-1, P-2, P-3, P-5, and P-7.

    Impact:

    • Highlights the environmental contamination risk from antibiotic manufacturing effluent.
    • Demonstrates the potential for horizontal gene transfer of antibiotic resistance in environmental bacteria.
    • Provides data on plasmid diversity and transferability, crucial for understanding antibiotic resistance spread.

    More Related Videos

    High-Resolution Comparison of Bacterial Conjugation Frequencies05:18

    High-Resolution Comparison of Bacterial Conjugation Frequencies

    Published on: January 10, 2019

    Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection08:34

    Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

    Published on: January 8, 2020

    Related Concept Videos

    Antibiotic Selection01:45

    Antibiotic Selection

    Researchers use antibiotic resistance genes to identify bacteria that possess a plasmid containing their gene of interest. Antibiotic resistance naturally occurs when a spontaneous DNA mutation creates changes in bacterial genes that eliminate antibiotic activity. Bacteria can share these new resistance genes with their offspring and other bacteria. The overuse and misuse of antibiotics have created a public health crisis, as resistant and multi-resistant bacteria continue to develop.Antibiotic...
    Plasmids01:28

    Plasmids

    Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
    Bacterial Phylum Proteobacteria01:26

    Bacterial Phylum Proteobacteria

    Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
    Bacterial Phylum Planctomycetes01:26

    Bacterial Phylum Planctomycetes

    Planctomycetes are a group of morphologically distinct bacteria predominantly classified into two orders: Planctomycetales and Brocadiales. These gram-negative bacteria exhibit unique features, including division by budding and the presence of stalks or appendages. Their cells are often found in rosette arrangements, and they are notable for possessing an S-layer in their cell envelope, which is relatively uncommon among bacteria. Additionally, Planctomycetes frequently exhibit intracellular...
    Bacterial Phylum Tenericutes01:24

    Bacterial Phylum Tenericutes

    The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
    Bioplastics01:27

    Bioplastics

    Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...