Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

1 Alpha 2 alpha 3 alpha collagen is arthritogenic.

K Morgan, H B Evans, S A Firth

    Annals of the Rheumatic Diseases
    |December 1, 1983
    PubMed
    Summary

    Both type II collagen and the minor collagen 1 alpha 2 alpha 3 alpha are immunogenic and arthritogenic in rats. Arthritis development varied between rat strains, indicating strain-specific responses to these cartilage collagens.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    On a New and Simple Method for the Cure of Fistula.

    Western journal of medicine and surgery·2024
    Same author

    Detection of Treponema pallidum, Haemophilus ducreyi, and Herpes Simplex Virus by Multiplex PCR.

    Methods in molecular medicine·2011
    Same author

    Increased gelatinase activity in systemic sclerosis dermal fibroblast cultures with unaltered gelatinase A mRNA expression.

    Journal of dermatological science·2002
    Same author

    Jelly belly: a Drosophila LDL receptor repeat-containing signal required for mesoderm migration and differentiation.

    Cell·2001
    Same author

    Enhancing the specificity of the COBAS AMPLICOR CT/NG test for Neisseria gonorrhoeae by retesting specimens with equivocal results.

    Journal of clinical microbiology·2001
    Same author

    Association of Mycoplasma genitalium with nongonococcal urethritis in heterosexual men.

    The Journal of infectious diseases·2000

    Area of Science:

    • Biochemistry
    • Immunology
    • Rheumatology

    Background:

    • Cartilage contains multiple collagen types, with type II being the major component.
    • The role of minor collagen components in cartilage, such as 1 alpha 2 alpha 3 alpha collagen, in immune responses and arthritis is less understood.

    Purpose of the Study:

    • To investigate the immunogenicity and arthritogenicity of native 1 alpha 2 alpha 3 alpha collagen compared to native type II collagen in different rat strains.
    • To characterize the inflammatory polyarthritis induced by these collagens.

    Main Methods:

    • Administration of native 1 alpha 2 alpha 3 alpha collagen and native type II collagen (500 micrograms per rat) to Alderley Park and Sprague-Dawley rats.
    • Assessment of arthritis development, clinical appearance, onset, and histology.

    Related Experiment Videos

  • Evaluation of antibody cross-reactivity between the two collagen types.

    • Main Results:

    • Native 1 alpha 2 alpha 3 alpha collagen was immunogenic in both rat strains and arthritogenic in Alderley Park rats (46% arthritis).
    • Native type II collagen was immunogenic and arthritogenic in both strains (64% and 57% arthritis, respectively).
    • The induced polyarthritis showed similar clinical and histological features for both collagens, with strain-dependent differences in arthritogenicity.

    Conclusions:

    • Both the major (type II) and a minor (1 alpha 2 alpha 3 alpha) collagen components of cartilage are capable of inducing an immune response and arthritis in rats.
    • Rat strain differences significantly influence the arthritogenic potential of these collagens.
    • Cross-reactivity between antibodies to type II and 1 alpha 2 alpha 3 alpha collagen suggests shared epitopes.