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Changes in Leydig cell function during sexual maturation in the mouse.

D J Chase, A H Payne

    Biology of Reproduction
    |December 1, 1983
    PubMed
    Summary
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    Mouse Leydig cell androgen production changes significantly with age. Testosterone production peaks at 45 days, while androstanediol declines after 30 days, altering the testosterone to androstanediol ratio.

    Area of Science:

    • Reproductive Biology
    • Endocrinology
    • Andrology

    Background:

    • Leydig cells are crucial for androgen synthesis in testes.
    • Understanding Leydig cell function is vital for male reproductive health.
    • Age-related changes in Leydig cells impact hormone production.

    Purpose of the Study:

    • To investigate age-dependent changes in androgen production by mouse Leydig cells.
    • To quantify testosterone and androstanediol production from 20 to 60 days of age.
    • To compare Leydig cell sensitivity and maximal androgen output across different ages.

    Main Methods:

    • Isolation and purification of mouse Leydig cells using mechanical dissociation and metrizamide density gradients.
    • Incubation of Leydig cells with varying concentrations of human chorionic gonadotropin (hCG).

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  • Quantification of testosterone and androstanediol levels via radioimmunoassay.

    • Main Results:

    • Leydig cell sensitivity to hCG remained constant with age.
    • Maximum testosterone production increased significantly by 45 days and plateaued.
    • Androstanediol production peaked early (25 days) and declined, altering the testosterone/androstanediol ratio in favor of testosterone with age.

    Conclusions:

    • Mouse Leydig cell androgen production exhibits distinct age-related patterns, with testosterone becoming the predominant androgen post-puberty.
    • These findings provide insights into the developmental endocrinology of male reproduction.
    • The study highlights dynamic shifts in androgen metabolism during Leydig cell maturation.