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Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
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Cell-cell interaction and polarity of epithelial cells: specific perturbation using a monoclonal antibody

B A Imhof, H P Vollmers, S L Goodman

    Cell
    |December 1, 1983
    PubMed

    Insights

    Researchers identified a new cell-surface protein crucial for epithelial cell adhesion using a monoclonal antibody. This discovery advances understanding of cell-cell contact regulation in epithelial tissues.

    Area of Science:

    • Cell Biology
    • Immunology
    • Biochemistry

    Background:

    • Cell-cell adhesion is vital for maintaining tissue structure and function.
    • Understanding the molecular mechanisms of cell adhesion is crucial for various biological processes and diseases.

    Purpose of the Study:

    • To identify novel molecules involved in specific cell-cell contacts of Madin-Darby canine kidney (MDCK) epithelial cells.
    • To characterize the function and properties of a newly identified cell-surface antigen using a monoclonal antibody.

    Main Methods:

    • Selection of a monoclonal antibody (anti-Arc-1) using functional assays to perturb cell-cell contacts.
    • Characterization of the antigen using radioisotope labeling, immunoscanning electron microscopy, EDTA extraction, Western blotting, and gel filtration.

    Main Results:

    • The anti-Arc-1 antibody disrupted tight junctions, uncoupled MDCK cells, and abolished cell polarity.
    • The antigen is a cell-surface protein of MDCK cells, with homogeneous surface distribution and extractable by EDTA.
    • Antigenicity was observed at 130 and 40 kDa on Western blots and via gel filtration.

    Conclusions:

    • The hybridoma technique combined with functional screening is effective for identifying novel molecules in cell-cell adhesion.
    • The identified antigen plays a significant role in maintaining epithelial cell integrity and polarity.

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