Subcellular localization of human heart atypical creatine kinase

Human heart subcellular fractions were obtained using method A described by Scholte et al [7,8]. It was found that 3.5 +/- 2.5%, 3.4 +/- 1.9%, 8.5 +/- 2.9%, 2.5 +/- 1.3%, and 88 +/- 21% of the total creatine kinase activity was associated with the myofibrillar, nuclear, mitochondrial, microsomal and particle-free soluble fractions respectively. Using DEAE-sephacel chromatography it was determined that the atypical creatine kinase (CK-Z) accounted for approximately 90% of the creatine kinase activity associated with the mitochondrial fraction.