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Fasting modulates creatine entry into skeletal muscle in the mouse
Experientia
|December 15, 1983
Summary
Creatine uptake into mouse skeletal muscle is reduced by dietary creatine or fasting. Fasting also increases plasma creatine levels, suggesting muscle creatine entry is a regulated, saturable process.
Area of Science:
- Muscle physiology
- Nutritional science
- Biochemistry
Background:
- Creatine is an essential metabolite for energy production in skeletal muscle.
- Understanding creatine transport mechanisms is crucial for sports nutrition and therapeutic applications.
Purpose of the Study:
- To investigate the regulation of creatine entry into skeletal muscle.
- To determine the effect of creatine feeding and fasting on muscle creatine uptake and plasma creatine levels.
Main Methods:
- Mice were administered radiolabeled [14C]-creatine.
- Muscle creatine uptake and plasma creatine specific activity were measured under conditions of creatine feeding and fasting.
Main Results:
- [14C]-Creatine uptake into skeletal muscle was inhibited by both creatine feeding and fasting.
- Plasma creatine specific activity decreased with creatine feeding and increased with fasting.
Conclusions:
- Skeletal muscle creatine uptake is mediated by a saturable transport system.
- This creatine transport process is modulated by nutritional status, specifically fasting and creatine availability.