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Dimethyl sulfoxide inhibits phagocyte influx into infected pleural spaces and phagocyte locomotion in vitro
Abstract:
Influx of polymorphonuclear leukocytes (PMNs) and monocytes (MNs) into pleural spaces was decreased in dimethyl sulfoxide (DMSO)-treated rabbits infected intrapleurally with Staphylococcus aureus. In addition, pleural fluids contained S. aureus longer and marked pleural thickening with fibrosis occurred in DMSO-treated rabbits. DMSO also inhibited stimulated locomotion of PMN and MN in vitro, suggesting that the aforementioned responses in vivo may have occurred because of DMSO-mediated inhibition of the locomotion of PMN and MN.
Insights
Dimethyl sulfoxide (DMSO) impaired the movement of immune cells into infected pleural spaces in rabbits. This led to prolonged Staphylococcus aureus infection and increased pleural fibrosis.
Area of Science:
- Immunology
- Microbiology
- Pathology
Background:
- Staphylococcus aureus infection can cause pleural inflammation.
- Polymorphonuclear leukocytes (PMNs) and monocytes (MNs) are crucial immune cells involved in clearing bacterial infections.
- Dimethyl sulfoxide (DMSO) is a chemical compound with various biological effects.
Purpose of the Study:
- To investigate the effect of DMSO on the host immune response to Staphylococcus aureus-induced pleural infection in rabbits.
- To determine if DMSO affects the migration of PMNs and MNs to the pleural space.
- To assess the impact of DMSO on bacterial clearance and pleural tissue changes.
Main Methods:
- Rabbits were infected intrapleurally with Staphylococcus aureus.
- Some rabbits received DMSO treatment.
- Immune cell influx, bacterial load, and pleural tissue morphology were evaluated.
Main Results:
- DMSO treatment significantly reduced the influx of PMNs and MNs into the pleural space.
- Staphylococcus aureus persisted longer in the pleural fluid of DMSO-treated rabbits.
- DMSO-treated rabbits exhibited marked pleural thickening and fibrosis.
Conclusions:
- DMSO inhibits the migration of PMNs and MNs to sites of infection.
- DMSO treatment exacerbates Staphylococcus aureus pleural infection and promotes fibrotic responses.
- DMSO-mediated inhibition of leukocyte locomotion may underlie the observed adverse effects in vivo.