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Differences in urinary monochlorobenzene metabolites between rats and humans
International Archives of Occupational and Environmental Health
|January 1, 1983
Summary
This study details a liquid chromatography method to measure monochlorobenzene metabolites in urine. 4-chlorocatechol conjugates are identified as a reliable biomarker for occupational exposure.
Area of Science:
- Environmental Chemistry
- Toxicology
- Analytical Chemistry
Background:
- Monochlorobenzene (MCB) exposure can occur occupationally.
- Assessing MCB exposure requires reliable methods to detect its metabolites in biological samples.
Purpose of the Study:
- To describe a high-performance liquid chromatographic (HPLC) method for determining p-chlorobenzene mercapturic acid and 4-chlorocatechol conjugates in urine.
- To investigate differences in MCB urinary excretion patterns between rats and humans.
- To identify a suitable biomarker for monitoring MCB exposure in humans.
Main Methods:
- Urine samples were extracted and analyzed using HPLC.
- Urinary mercapturic acid was determined after benzene extraction.
- Urinary 4-chlorocatechol conjugates were measured after methanol dissolution and hydrolysis.
Main Results:
- Metabolite excretion in rats was proportional to administered doses.
- The ratio of mercapturic acid to 4-chlorocatechol varied across species and administration routes.
- Humans showed significantly lower p-chlorophenylmercapturic acid excretion compared to 4-chlorocatechol after oral or inhalation exposure.
Conclusions:
- The HPLC method effectively quantifies MCB metabolites.
- 4-chlorocatechol conjugate levels in urine serve as a suitable index for monitoring monochlorobenzene exposure in occupationally exposed individuals.