[Morphologic, endocrine and technical factors in relation to the results of human embryo transfer to the uterus]

Out of 161 cycles in which the procedure was attempted, 20 clinical pregnancies were obtained by in vitro fertilization and embryo transplant. The authors think that among the numerous parameters that can affect the result favourably are: At the time of oocyte recovery large follicles (23 mm) following treatment with Clomid gave oocytes with pregnancy following. Medium B2 added to human serum gave high fertilization rates (80%) and cell division rates (75%) following in vitro culture. Only those embryos where cell division occurred quickly gave rise to pregnancies. As far as the transplant was concerned, the pregnancy rate rose from 12 to 30% according to whether one replaced 1 or 2 embryos in the uterus. Preliminary examination of the cervical canal indicated whether it should be dilated in cases where it was difficult to introduce the catheter. Giving valium made the uterus relax whereas adding blood serum (50 to 75%) in the transfer medium increased the viscosity of this latter. 19 of the 20 pregnancies resulted when the embryo transfer was easily accomplished.