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Oleic acid transfer from microsomes to egg lecithin liposomes: participation of fatty acid binding protein
Abstract:
Oleic acid transfer from microsomes or mitochondria to egg lecithin liposomes was stimulated by fatty acid binding protein. By gel filtration, it could be demonstrated that this protein incorporates oleic acid into liposomes. Fatty acid binding protein transfer activity was higher using microsomes rather than mitochondria, which suggests a selective interaction with different kinds of membranes. Transfer of oleic acid by this soluble protein is greater than that of stearic acid. The results indicate that fatty acid binding protein may participate in the intracellular transport of fatty acids.
Insights
Fatty acid binding protein facilitates oleic acid transfer into liposomes, showing higher activity with microsomes than mitochondria. This suggests a role in intracellular fatty acid transport.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Transport
Background:
- Fatty acids are essential cellular components requiring regulated transport.
- Soluble proteins like fatty acid binding protein (FABP) are implicated in lipid metabolism.
Purpose of the Study:
- To investigate the role of fatty acid binding protein in the transfer of oleic acid to liposomes.
- To determine the selectivity of FABP for different membrane types and fatty acids.
Main Methods:
- Utilized gel filtration to analyze oleic acid incorporation into liposomes.
- Compared FABP-mediated oleic acid transfer from both microsomal and mitochondrial sources.
- Assessed the transfer of oleic acid versus stearic acid by FABP.
Main Results:
- Fatty acid binding protein significantly stimulated oleic acid transfer to egg lecithin liposomes.
- FABP demonstrated higher transfer activity from microsomes compared to mitochondria, indicating membrane selectivity.
- Oleic acid transfer by FABP was more efficient than stearic acid transfer.
Conclusions:
- Fatty acid binding protein actively incorporates oleic acid into liposomes.
- FABP exhibits selective interaction with different cellular membranes.
- Results support the hypothesis that FABP plays a role in intracellular fatty acid transport.