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Effect of dimethoate and O-demethyldimethoate on bone marrow cells of CFLP mice
Abstract:
The organophosphorus pesticide dimethoate and its nonalkylating O-demethyl derivative were tested for their ability to induce chromosomal alterations in bone marrow cells of CFLP mice after ip administration. A single dose of 20 mg/kg dimethoate proved to be ineffective. However, doses of 60 mg/kg dimethoate or 69 mg/kg O-demethyldimethoate sodium salt significantly increased the aberration rates above those of the controls. The same effect was observed after a nontoxic dose of 690 mg/kg O-demethyldimethoate sodium salt. Considering the distribution of the several aberration types, these findings suggest that the alkylating properties of dimethoate only in part may be responsible for its cytogenetic activity.
Insights
Organophosphorus pesticide dimethoate and its O-demethyl derivative induced chromosomal alterations in mice bone marrow cells. Higher doses of dimethoate and O-demethyldimethoate showed significant genotoxicity, suggesting partial alkylating activity.
Area of Science:
- Environmental toxicology
- Genetics and heredity
- Pesticide research
Background:
- Organophosphorus pesticides are widely used in agriculture.
- Dimethoate is a common insecticide with potential health risks.
- Cytogenetic alterations are indicators of genotoxicity.
Purpose of the Study:
- To evaluate the genotoxic potential of dimethoate and its O-demethyl derivative.
- To investigate the role of alkylating properties in dimethoate's cytogenetic activity.
Main Methods:
- Bone marrow cells of CFLP mice were analyzed for chromosomal aberrations.
- Mice were administered single intraperitoneal doses of dimethoate or O-demethyldimethoate sodium salt.
- Aberration rates were compared between treated groups and controls.
Main Results:
- A single dose of 20 mg/kg dimethoate did not induce significant chromosomal alterations.
- Doses of 60 mg/kg dimethoate and 69 mg/kg O-demethyldimethoate sodium salt significantly increased aberration rates.
- A nontoxic dose of 690 mg/kg O-demethyldimethoate sodium salt also elevated aberration rates.
Conclusions:
- Dimethoate and its O-demethyl derivative exhibit genotoxic effects, inducing chromosomal aberrations in mice.
- The alkylating properties of dimethoate contribute to, but do not fully explain, its cytogenetic activity.
- Further research is needed to elucidate the mechanisms of dimethoate-induced genotoxicity.