Study of chromosomal replication of red muntjac (Muntiacus muntjac)

The technique of cell fusion, the method of sister chromatid differentiation (SCD), and the silver staining method modified by us that can show nucleolus organizing region (NOR) and sister chromatid differentiation at the same time have been used in the study of red muntjac chromosomes. Differential staining of sister chromatid has been observed in metaphase chromosomes and in premature condensed chromosomes (PCC) at various stages of the interphase. Our silver staining method shows that the NOR in the dark staining chromatid is Ag-positive, while that in the light staining chromatid is Ag-negative. The chromatid differentiation staining and the NOR sister staining suggest that the structure of metaphase chromosomes is similar to that of the premature condensed chromosomes of the interphase cell. The chromosomal replication of red muntjac conforms with the semiconservative replication mechanism and the replication of rDNA is also semiconservative.