Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Coagulation testing with the Du Pont aca discrete clinical analyzer.

J F Pierson-Perry, J A Wehrly, G E Siefring

    Seminars in Thrombosis and Hemostasis
    |October 1, 1983
    PubMed
    Summary

    New automated methods for measuring Antithrombin III (AT III), Plasminogen (PLG), and Fibrinogen (FBG) offer excellent reproducibility for hemostatic function evaluation. These advancements aid in standardizing coagulation testing in hospital laboratories.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sensitive immunoassay for human immunodeficiency viral core proteins.

    Methods in enzymology·1990
    Same author

    Development and characterization of an automated assay of effective heparin activity in plasma.

    Clinical chemistry·1987
    Same author

    Enzymatic modifications of human plasma fibronectin in relation to opsonizing activity.

    Biochemistry·1983
    Same author

    New colored and fluorescent amine substrates for activated fibrin stabilizing factor (Factor XIIIa) and for transglutaminase.

    Analytical biochemistry·1983
    Same author

    Degradation of transmembrane proteins in Ca2+-enriched human erythrocytes. An immunochemical study.

    The Journal of biological chemistry·1983
    Same author

    Development and analytical performance of a functional assay for fibrinogen on the Du Pont aca analyzer.

    Clinical chemistry·1983

    Area of Science:

    • Clinical chemistry
    • Hematology
    • Coagulation diagnostics

    Background:

    • Accurate measurement of coagulation factors is crucial for assessing hemostatic function.
    • Existing methodologies may lack automation or sufficient reproducibility.
    • The Du Pont aca discrete clinical analyzer is a widely used platform in hospital laboratories.

    Purpose of the Study:

    • To develop and validate automated methods for measuring Antithrombin III (AT III), Plasminogen (PLG), and Fibrinogen (FBG) on the Du Pont aca analyzer.
    • To assess the reproducibility and comparability of these new methods.
    • To evaluate the potential of these assays for improving hemostasis testing and standardization.

    Main Methods:

    • Development of novel assay methodologies for AT III, PLG, and FBG.

    Related Experiment Videos

  • Implementation of these methods on the Du Pont aca discrete clinical analyzer.
  • Validation of assay performance including reproducibility and comparison with established techniques.

    • Main Results:

    • Fully automated methods for AT III, PLG, and FBG measurement were successfully developed.
    • The developed methods demonstrated excellent reproducibility.
    • Comparative analysis indicated favorable agreement with existing methodologies.

    Conclusions:

    • Automated assays for AT III, PLG, and FBG are now available for the Du Pont aca analyzer.
    • These methods provide reliable hemostatic function evaluation for hospital laboratories.
    • High reproducibility and the use of reference standards will advance the standardization of coagulation assays.