[Determination of the influenza virus concentration in chromatographic vaccinal preparations]

Concentration of influenza virus and its surface antigens in inactivated chromatographic vaccines was evaluated to an accuracy of 10% by determinations of total protein concentrations in them. This approach to the evaluation of virus concentration is possible because in the currently released inactivated chromatographic influenza vaccines at least 90% of the total protein consist of virus particle protein. This is based on the analysis of virus vaccines made by polyacryl amide gel-sodium dodecyl sulphate electrophoresis. It was calculated that 3 X 10(11) virus particles in 1 ml correspond to 100 micrograms/ml virus protein (approximate concentration of protein in vaccine preparations).