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Inactivation of regulatory volume decrease in human peripheral blood lymphocytes by N-ethylmaleimide.

J Bauer, P K Lauf

    Biochemical and Biophysical Research Communications
    |November 30, 1983
    PubMed
    Summary
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    N-ethylmaleimide inhibits regulatory volume decrease in human lymphocytes by affecting ion transport. This sulfhydryl reagent prevents secondary swelling in hypotonic conditions, unlike its effect on red blood cells.

    Area of Science:

    • Cellular physiology
    • Ion transport mechanisms
    • Biochemistry

    Background:

    • Human peripheral lymphocytes regulate their volume in response to osmotic stress.
    • Ion transport is crucial for cellular volume homeostasis.

    Purpose of the Study:

    • To investigate the effect of N-ethylmaleimide (NEM) on lymphocyte volume regulation.
    • To elucidate the role of sulfhydryl groups in ion transport during volume changes.

    Main Methods:

    • Human peripheral lymphocytes were swollen in hypotonic NaCl and KCl media.
    • The effect of N-ethylmaleimide (NEM) on regulatory volume decrease (RVD) was measured.
    • Dose-dependent inhibition by NEM was assessed.

    Main Results:

    Related Experiment Videos

  • N-ethylmaleimide inhibited regulatory volume decrease in a dose-dependent manner.
  • NEM-treated lymphocytes showed reduced secondary swelling in hypotonic KCl.
  • This contrasts with NEM's stimulatory effect on K/Cl fluxes in red cells.

    • Conclusions:

    • N-ethylmaleimide likely targets ion transport mechanisms involved in lymphocyte volume regulation.
    • The data suggest differential effects of NEM on ion transport in lymphocytes versus red blood cells.