Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Exodeoxyribonuclease from rat brain specific for single-stranded DNA.

V A Ivanov, A I Gaziev, T M Tretyak

    European Journal of Biochemistry
    |December 15, 1983
    PubMed
    Summary

    Researchers isolated and purified a single-stranded DNA-specific exodeoxynuclease from rat brain. This enzyme, found in neuronal nuclei, hydrolyzes various DNA substrates but not UV-irradiated ones.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Triple valve replacement after recurrent infective endocarditis and previous COVID-19 infection].

    Khirurgiia·2025
    Same author

    [Long-term results of multivalvular correction of heart defects].

    Khirurgiia·2025
    Same author

    [Adherent-invasive Escherichia <i>coli</i> isolated from a Crohn's disease patient exhibits colonization capacity and pro-inflammatory effects in the murine intestine].

    Voprosy pitaniia·2025
    Same author

    [Immediate and long-term results of the simultaneous rhythm-converting procedure Cox-Maze IV in multivalvular correction].

    Khirurgiia·2024
    Same author

    [Adapted scale for analysis of necessary septal myectomy in aortic valve replacement].

    Khirurgiia·2024
    Same author

    Therapeutic Effects of Bacteroides fragilis Vesicles in a Model of Chemically Induced Colitis in Rats.

    Bulletin of experimental biology and medicine·2024

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Neuroscience

    Background:

    • Exodeoxynucleases play crucial roles in DNA metabolism and repair.
    • Understanding the specificities and localizations of nucleases is vital for comprehending cellular processes.

    Purpose of the Study:

    • To isolate and characterize a novel exodeoxynuclease from rat brain tissue.
    • To determine the substrate specificity, cofactor requirements, and cellular localization of the purified enzyme.

    Main Methods:

    • Purification of the exodeoxynuclease using a combination of chromatography techniques (DNA-agarose, hydroxyapatite, Sephadex G-200).
    • Biochemical characterization including pH optimum, cofactor requirements (Mg2+ or Mn2+), and molecular weight determination (approx. 60,000).
    • Enzyme activity assays using various DNA substrates (homologous, heterologous, synthetic, depurinated, UV-irradiated).

    Related Experiment Videos

  • Cellular localization studies using differential tissue analysis (neuronal nuclei vs. liver tissue).

    • Main Results:

    • An exodeoxynuclease with single-stranded DNA specificity was purified 1100-fold from rat brain.
    • The enzyme exhibits optimal activity at pH 8.4, requires divalent cations (Mg2+ or Mn2+), and has a molecular weight of ~60,000.
    • It efficiently hydrolyzes various DNA substrates into 5'-monophosphates but is inactive against UV-irradiated polydeoxyribonucleotides.
    • The DNase is primarily localized in neuronal cell nuclei and is notably absent in rat liver tissue.

    Conclusions:

    • A novel single-stranded DNA-specific exodeoxynuclease has been identified and purified from rat brain.
    • This enzyme's unique substrate specificity and predominant neuronal nuclear localization suggest a specialized role in neuronal DNA metabolism or repair.
    • Further investigation into this enzyme's function could provide insights into neuronal biology and potential neurological disease mechanisms.