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Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
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[Spirochetes: coiling direction].

A Kayser, M Adrian

    Annales De Microbiologie
    |April 1, 1978
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    Summary
    This summary is machine-generated.

    Spirochetes, including leptospires, exhibit a right-handed coil, contrary to common depictions. This helical structure is revealed by observing the alternating visibility of their axial fibers in electron microscopy.

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    Area of Science:

    • Microbiology
    • Microscopy
    • Cell Biology

    Context:

    • Electron microscopy reveals the complex structure of spirochetes.
    • Axial fiber visibility in spirochetes offers clues to their morphology.
    • Published images often misrepresent the true helical nature of spirochetes.

    Purpose:

    • To determine the true helical sense of spirochetes using electron microscopy.
    • To explain the discrepancy between observed and published spirochete structures.
    • To correct the common misrepresentation of spirochete coiling.

    Summary:

    • Observation of axial fibers alternating above and below the cytoplasmic cylinder in electron micrographs.
    • Inference of a right-handed helical structure for leptospires and other spirochetes.
    • Explanation for reversed imaging in published micrographs and drawings due to preparation and viewing angles.

    Impact:

    • Corrects the prevailing misconception of spirochete morphology.
    • Provides accurate visualization of spirochete helical structure.
    • Enhances understanding of bacterial motility and structure in microbiology.