[Study of the method of direct immunofluorescence for differentiation of serotypes of Trichomonas]

It is possible to differentiate the serotypes of Trichomonas vaginalis by the direct immunofluorescent method using the tupe-specific antisera labelled with fluorescin-isothiocyanate. The most decisive factor in expressing the degree of induced fluorescence is the serotype of the protozoa. Evans blue, which has an intensive red fluorescence, totally eliminates the green autofluorescence of T. vaginalis and does not influence the induced specific fluorescence.