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Augmentation of natural killer activity of human peripheral blood lymphocytes by human leukocyte interferon:
The characteristics of natural killer (NK) activity of human peripheral blood lymphocytes treated with human leukocyte interferon (HuIFN-alpha) were compared with those of the untreated original NK activity. HuIFN-alpha, at a concentration of 50 IU/ml or more, augmented the NK activity against BALL-1, Daudi, and Namalwa target cells. On the other hand, the augmentation was less evident for the activity against Raji and Molt-4 cells. Original NK activity was detected mainly in the fraction that passed through a nylon wool column. Nylon-retained cells, however, could be activated by the addition of HuIFN-alpha to express activity against BALL-1. The activity of the nylon-passed fraction was augmented by HuIFN-alpha as well as that of unfractionated cells. NK cells could be activated by HuIFN-alpha under calcium-free conditions that allow NK cells to bind but not to lyse the target cells; augmented cytotoxicity was observed when calcium was added 2 hr later. This suggests that the augmentation of NK activity by HuIFN-alpha may have resulted from an increase in the number of NK cells.
The characteristics of natural killer (NK) activity of human peripheral blood lymphocytes treated with human leukocyte interferon (HuIFN-alpha) were compared with those of the untreated original NK activity. HuIFN-alpha, at a concentration of 50 IU/ml or more, augmented the NK activity against BALL-1, Daudi, and Namalwa target cells. On the other hand, the augmentation was less evident for the activity against Raji and Molt-4 cells. Original NK activity was detected mainly in the fraction that passed through a nylon wool column. Nylon-retained cells, however, could be activated by the addition of HuIFN-alpha to express activity against BALL-1. The activity of the nylon-passed fraction was augmented by HuIFN-alpha as well as that of unfractionated cells. NK cells could be activated by HuIFN-alpha under calcium-free conditions that allow NK cells to bind but not to lyse the target cells; augmented cytotoxicity was observed when calcium was added 2 hr later. This suggests that the augmentation of NK activity by HuIFN-alpha may have resulted from an increase in the number of NK cells.