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Molar antithrombin concentration in normal human plasma.

J Conard, F Brosstad, M Lie Larsen

    Haemostasis
    |January 1, 1983
    PubMed
    Summary
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    Human plasma inactivates thrombin, with heparin affecting this process differently for crude versus purified thrombin. This study quantifies antithrombin concentration in reference plasma.

    Area of Science:

    • Biochemistry
    • Hematology

    Background:

    • Thrombin is a key enzyme in hemostasis.
    • Human plasma contains inhibitors that regulate thrombin activity.
    • Antithrombin is a primary inhibitor of thrombin.

    Purpose of the Study:

    • To quantify the thrombin-inactivating capacity of human plasma.
    • To investigate the effect of heparin on plasma's thrombin-inactivating ability.
    • To determine the concentration of antithrombin in reference plasma.

    Main Methods:

    • Incubation of crude and purified bovine thrombin with human plasma.
    • Measurement of inactivated thrombin using NIH units.
    • Active site titration for purified thrombin molar concentration determination.
    • Comparison of thrombin inactivation with and without heparin.

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    Main Results:

    • Human plasma inactivated 140-193 NIH units of crude thrombin per ml.
    • Heparin reduced plasma's inactivating capacity for crude thrombin, but less so for purified thrombin.
    • Purified bovine thrombin was 92% pure.
    • Plasma inactivated 2.57 mumol/l of thrombin without heparin and 2.50 mumol/l with heparin.

    Conclusions:

    • Plasma's thrombin inactivation is influenced by thrombin preparation purity and heparin presence.
    • The concentration of antithrombin in pooled reference plasma is approximately 2.57 mumol/l (0.15 g/l).