Dielectric properties of murine lymphocytes

Dielectric measurements in frequency range from 0.032 up to 110 MHZ were performed on the suspension of lymphocytes isolated from the spleen of Balb/c mouse. Basing on measurements of electric capacitance (C) and electric conductance (G) of lymphocyte suspension and on the simplified Pauly-Schwan equations the following electrical parameters of lymphocytes were calculated: relative electrical permittivity of the intracellular substance (epsilon i = 136) electrical conductivity of the inner phase (intracellular substance) (kappa i = 8.94 mS/cm), specific capacitance of cell membrane (CM = 0.82 microF/cm2). Relative electrical permittivity of the membrane (epsilon s = 4.6) has also been calculated. Two of those quantities (epsilon i = and kappa i) additionally determined by means of empirical dependences. The value of epsilon i and kappa i were respectively 125 and 8.79 mS/cm. All those measurements were taken at temperature amounting to 293 K.