Biochemical, hematological, and histological changes in B6C3F1 mice after intramuscular administration of PROven

Repeated intramuscular administration of PROven to B6C3F1 mice has a potential cardiotoxic effect, an immunogenic effect, and a hematological effect. A significant decrease in two lactic dehydrogenase isoenzymes of myocardial origin was a result of chronic administration of PROven. The immunological effect was observed as a significant increase in beta- and gamma-globulins. The hematological effect of PROven was to increase the number of circulating neutrophils. Both the immunogenic and hematological effect should be anticipated due to the foreign protein nature of PROven. Following intramuscular injections of PROven, severe inflammatory changes including necrosis of skeletal muscle tissue were seen histologically.