An improved purification procedure and apoprotein preparation for the flavodoxin from Azotobacter vinelandii

An improved method for the purification of the holoflavodoxin from Azotobacter vinelandii was developed, which resulted in improved yields and degree of homogeneity. The purity and homogeneity of this sample were established by polyacrylamide gel electrophoresis. An apoprotein preparation procedure is outlined, which results in a homogeneous preparation of the apoflavodoxin. The homogeneity of the apoflavodoxin sample was established by polyacrylamide gel electrophoresis.